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(B) $DNA$ replication is semi-conservative in nature.When $E. coli$ cells are grown in a medium containing radioactive thymidine,the newly synthesized

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One strand will be radioactive.

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(B) $DNA$ replication is semi-conservative in nature.When $E. coli$ cells are grown in a medium containing radioactive thymidine,the newly synthesized $DNA$ strands incorporate the radioactive label.After $5$ minutes,the $DNA$ molecules will have one radioactive strand (template) and one newly synthesized radioactive strand (if replication occurred) or just the labeled template.When these cells are transferred to a normal medium,the new strands synthesized will use non-radioactive nucleotides.Since the original $DNA$ molecule had one radioactive strand and one non-radioactive strand (or both radioactive depending on the duration),the resulting daughter $DNA$ molecules will retain the radioactive strand as a template.Specifically,in this experiment,one strand of the $DNA$ duplex will remain radioactive because it serves as the template for the new,non-radioactive strand.Therefore,one strand of the $DNA$ will be radioactive.

$E. coli$ cells were allowed to replicate in a medium containing radioactive thymidine for $5$ minutes and then transferred to a normal medium for further replication. Which of the following observations will be correct?

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