Which of the following is NOT required for th | vedclass

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(A) $DNA$ fingerprinting involves identifying differences in specific regions of $DNA$ sequence called repetitive $DNA$. The steps involved are: $1$.

Vedclass · Accepted Answer

Zinc finger analysis

Vedclass · Answer

(A) $DNA$ fingerprinting involves identifying differences in specific regions of $DNA$ sequence called repetitive $DNA$. The steps involved are: $1$. Isolation of $DNA$. $2$. Digestion of $DNA$ by restriction endonucleases. $3$. Separation of $DNA$ fragments by electrophoresis. $4$. Transferring (blotting) of separated $DNA$ fragments to synthetic membranes,such as nitrocellulose or nylon. $5$. Hybridization using labelled $VNTR$ probe. $6$. Detection of hybridized $DNA$ fragments by autoradiography. Polymerase chain reaction $(PCR)$ is often used to amplify the $DNA$ sample if the amount of $DNA$ is very small. Zinc finger analysis is a technique used for studying $DNA$-binding proteins and is not a part of the standard $DNA$ fingerprinting procedure.

Which of the following is $NOT$ required for the current $DNA$ fingerprinting technique?

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