In the context of blue-white screening,non-re | vedclass

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(B) The blue-white screening method is based on the insertional inactivation of the $lacZ$ gene. $1$. The $lacZ$ gene encodes the enzyme $\beta$-galac

Vedclass · Accepted Answer

Non-recombinant bacteria contain beta-galactosidase.

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(B) The blue-white screening method is based on the insertional inactivation of the $lacZ$ gene. $1$. The $lacZ$ gene encodes the enzyme $\beta$-galactosidase. $2$. In non-recombinant bacteria,the $lacZ$ gene is intact and functional,so the bacteria produce $\beta$-galactosidase. This enzyme reacts with the chromogenic substrate $X-gal$ to produce a blue color. $3$. In recombinant bacteria,the foreign $DNA$ is inserted into the $lacZ$ gene,causing insertional inactivation. Consequently,no functional $\beta$-galactosidase is produced,and the colonies remain white.

In the context of blue-white screening,non-recombinant bacterial colonies appear blue in contrast to white recombinant colonies because:

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