Explain $DNA$ fingerprinting in detail.

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(N/A) The technique of identifying the specific sequence of nucleotides in $DNA$ that is unique to each individual is known as $DNA$ fingerprinting.
To understand the genetic differences between two individuals or populations,one must know the $DNA$ sequence.
$DNA$ fingerprinting is a rapid method for comparing the sequences of two individuals.
In $DNA$ fingerprinting,differences are identified in specific regions of the $DNA$ sequence known as repetitive $DNA$,where a small segment of $DNA$ is repeated many times.
This repetitive $DNA$ is separated from the bulk genomic $DNA$ by density gradient centrifugation. The bulk $DNA$ forms a major peak,while other small peaks are formed,which are called satellite $DNA$.
Based on base composition ($A:T$ or $G:C$ richness),segment length,and the number of repetitive units,they are classified into microsatellites,minisatellites,etc.
These sequences do not code for any proteins but constitute a large portion of the human genome. They exhibit a high degree of polymorphism,which is the basis of $DNA$ fingerprinting.
$DNA$ obtained from any tissue of an individual,such as blood,hair,skin,bones,saliva,or sperm,shows the same type of polymorphism. This is useful as a significant identification tool in forensic applications. This polymorphism is inherited from parents to offspring; therefore,it is an accurate method for resolving paternity disputes.

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Arrange the various steps of $DNA$ fingerprinting technique in the correct order.
$(I)$ Separation of $DNA$ fragment by electrophoresis.
$(II)$ Digestion of $DNA$ by restriction endonucleases.
$(III)$ Hybridization using labeled $VNTR$ probe.
$(IV)$ Isolation of $DNA$.
$(V)$ Detection of hybridized $DNA$ fragments by autoradiography.
$(VI)$ Transferring of separated $DNA$ fragment to synthetic membranes.

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In $DNA$ fingerprinting,detection of hybridised $DNA$ fragments is possible by

Arrange the following steps of $DNA$ fingerprinting in a correct sequence.
$A$. Isolation of $DNA$ and its digestion by restriction endonucleases.
$B$. Hybridisation using a labelled $VNTR$ probe.
$C$. Transferring of separated $DNA$ fragments to synthetic membranes.
$D$. Detection of hybridised $DNA$ fragments by autoradiography.
$E$. Separation of $DNA$ fragments by electrophoresis.
Choose the correct answer from the options given below:

Which of the following statements is incorrect?

Give scientific reasons: $DNA$ polymorphism is the basis of genetic mapping of human genome.

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