On what basis are DNA fragments separated in | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) Agarose gel electrophoresis is a technique used to separate $DNA$ fragments based on their size.Since $DNA$ molecules are negatively charged due t

Vedclass · Accepted Answer

Size

Vedclass · Answer

(A) Agarose gel electrophoresis is a technique used to separate $DNA$ fragments based on their size.Since $DNA$ molecules are negatively charged due to the phosphate backbone,they move towards the anode $(+)$ when an electric field is applied.The agarose gel acts as a molecular sieve.Smaller $DNA$ fragments move faster through the pores of the gel matrix compared to larger fragments,resulting in their separation based on size.

On what basis are $DNA$ fragments separated in agarose gel electrophoresis?

Similar Questions

During $PCR$, primers bind to the $DNA$ strands in the . . . . . . step.

$A$ recombinant $DNA$ molecule was created by ligating a gene to a plasmid vector. By mistake,an exonuclease was added to the tube containing the recombinant $DNA$. How does this affect the next step in the experiment,i.e.,bacterial transformation?

Biolistics (gene gun) is suitable for:

$A$ bioreactor is

$A$ simple stirred-tank bioreactor is shown below. Identify $A, B, C, D$,and $E$.

Vedclass Test Series

Exam Paper Generator

Online Exam Module