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(D) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The $BamHI$ r

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Medium containing tetracycline antibiotic

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(D) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The $BamHI$ restriction site is located within the $tet^R$ gene.When a foreign $DNA$ fragment is inserted into the $BamHI$ site,it causes insertional inactivation of the $tet^R$ gene.As a result,the recombinant $DNA$ molecule loses its resistance to tetracycline.Therefore,the recombinants will not grow in a medium containing tetracycline antibiotic.

If a foreign $DNA$ fragment is inserted into the $BamHI$ site of $pBR322$ to create $r-DNA$,in which medium will the recombinants not grow?

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