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(D) The Polymerase Chain Reaction $(PCR)$ is a technique used to amplify a specific segment of $DNA$. To perform $PCR$,the following components are es

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Template $DNA$,$Taq$ polymerase,primers,deoxyribonucleotides

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(D) The Polymerase Chain Reaction $(PCR)$ is a technique used to amplify a specific segment of $DNA$. To perform $PCR$,the following components are essential: $1$. Template $DNA$: The $DNA$ segment containing the gene of interest to be amplified. $2$. Primers: Small chemically synthesized oligonucleotides that are complementary to the regions of the $DNA$ template. $3$. $Taq$ polymerase: $A$ thermostable $DNA$ polymerase enzyme isolated from the bacterium $Thermus$ $aquaticus$,which remains active at high temperatures required for denaturation. $4$. Deoxyribonucleotides (dNTPs): The building blocks required for the synthesis of new $DNA$ strands. Therefore,option $D$ is the correct answer as it includes all the necessary components.

Which of the following is required for the amplification of a gene of interest by $PCR$?

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