How many polypeptide chains is human insulin composed of?

Correct chronological order of the steps occurring during gene therapy are
$I$. Lymphocytes are obtained from the patients
$II$. Lymphocytes are transferred to the culture dishes
$III$. Lymphocytes are transfected with the normal $ADA$ genes
$IV$. The transfected cells are returned to the patients
The chronological order should be

When and on whom was the first clinical gene therapy experiment performed?

How is a permanent cure for $ADA$ deficiency possible?

Which one of the following is the first genetically engineered medicine?

The Indian Parliament cleared which amendment of the Indian Patent Bill, which is significant in the context of biotechnology and intellectual property rights?