Which protein is used in the treatment of emphysema?

The first clinical gene therapy was given for treating

In the earlier times,the insulin used for diabetic patients was:

Correct chronological order of the steps occurring during gene therapy are
$I$. Lymphocytes are obtained from the patients
$II$. Lymphocytes are transferred to the culture dishes
$III$. Lymphocytes are transfected with the normal $ADA$ genes
$IV$. The transfected cells are returned to the patients
The chronological order should be

Applications,like bioremediation,processed food,therapeutics and diagnostics are related with

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?