PCR proceeds in three distinct steps governed | vedclass

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(D) single $PCR$ amplification cycle involves three basic steps: $1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx.

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Denaturation,annealing,synthesis (polymerization)

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(D) single $PCR$ amplification cycle involves three basic steps: $1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-95^{\circ}C$) to separate the two strands. $2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow the primers to bind to the complementary sequences on the single-stranded $DNA$ templates. $3$. Synthesis (Extension/Polymerization): The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ $DNA$ polymerase to extend the primers by adding nucleotides,resulting in the synthesis of new $DNA$ strands. Therefore,the correct order is Denaturation,Annealing,and Synthesis (polymerization).

$PCR$ proceeds in three distinct steps governed by temperature. They are in order of:

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