A: Selection of recombinants due to inactivat | vedclass

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(A) The selection of recombinant cells using antibiotic resistance genes (insertional inactivation) involves identifying cells that have lost their re

Vedclass · Accepted Answer

Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

Vedclass · Answer

(A) The selection of recombinant cells using antibiotic resistance genes (insertional inactivation) involves identifying cells that have lost their resistance to a specific antibiotic due to the insertion of foreign $DNA$.This process is considered cumbersome because it requires the replica plating technique,where the same set of colonies must be transferred onto two different plates: one containing the antibiotic for which the gene was inactivated and another containing a different antibiotic (or no antibiotic) to ensure the survival of the colonies.Since both the Assertion and the Reason are scientifically accurate and the Reason correctly explains why the procedure is considered cumbersome,the correct option is $A$.

$I$	$II$	$III$
$(a)$ $PCR$	$(i)$ Specific base sequence	$p.$ $Taq$ polymerase
$(b)$ Downstream processing	$(ii)$ Inheritable gene	$q.$ Ampicillin resistant
$(c)$ Restriction endonuclease	$(iii)$ Amplification of $DNA$	$r.$ $E. coli$
	$(iv)$ Product separation and modification	$s.$ Quality control

List-$I$	List-$II$
$(a)$ Gel electrophoresis	$(1)$ Amplification of gene of interest
$(b)$ $PCR$	$(2)$ Separation of $DNA$ fragments
$(c)$ Bioreactor	$(3)$ Plasmid
$(d)$ Cloning vector	$(4)$ Production of desired product

$A$: Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure.
$R$: It requires simultaneous plating on two plates having different antibiotics.

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Match the following items in List-$I$ with List-$II$:
List-$I$ List-$II$
$(a)$ Gel electrophoresis $(1)$ Amplification of gene of interest
$(b)$ $PCR$ $(2)$ Separation of $DNA$ fragments
$(c)$ Bioreactor $(3)$ Plasmid
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$A$: Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure.$R$: It requires simultaneous plating on two plates having different antibiotics.