A : Pst I generates single-stranded overhangi | vedclass

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(A) $Pst I$ is a restriction endonuclease that cuts $DNA$ at specific recognition sequences,creating single-stranded overhanging stretches known as 's

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Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

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(A) $Pst I$ is a restriction endonuclease that cuts $DNA$ at specific recognition sequences,creating single-stranded overhanging stretches known as 'sticky ends'.These sticky ends facilitate the ligation process because they can form hydrogen bonds with their complementary counterparts on another $DNA$ fragment.Once the hydrogen bonds are formed,the enzyme $DNA$ ligase seals the nicks by forming phosphodiester bonds,resulting in a continuous recombinant $DNA$ molecule.

$A$ : $Pst I$ generates single-stranded overhanging stretches in $DNA$ after digestion that facilitate ligation.
$R$ : These short extensions can form hydrogen bonds with their complementary counterparts,which are then sealed by $DNA$ ligase to form phosphodiester bonds.

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$A$ : $Pst I$ generates single-stranded overhanging stretches in $DNA$ after digestion that facilitate ligation.$R$ : These short extensions can form hydrogen bonds with their complementary counterparts,which are then sealed by $DNA$ ligase to form phosphodiester bonds.