A : Ethidium bromide helps in visualizing DNA | vedclass

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(A) Ethidium bromide $(EtBr)$ is a fluorescent dye used to stain $DNA$ fragments in agarose gel electrophoresis.When exposed to $UV$ light,the $DNA$ b

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Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

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(A) Ethidium bromide $(EtBr)$ is a fluorescent dye used to stain $DNA$ fragments in agarose gel electrophoresis.When exposed to $UV$ light,the $DNA$ bands stained with $EtBr$ fluoresce as bright orange bands.This occurs because $EtBr$ is an intercalating agent that binds between the base pairs of the $DNA$ double helix.It absorbs light in the $UV$ range,specifically between $260-330 \; nm$,and emits light in the visible spectrum (orange).Therefore,both the Assertion and the Reason are correct,and the Reason provides the correct explanation for why $DNA$ can be visualized under $UV$ light.

$A$ : Ethidium bromide helps in visualizing $DNA$ in $UV$ light only.
$R$ : This intercalating agent absorbs light in range $260-330 \; nm$.

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$A$ : Ethidium bromide helps in visualizing $DNA$ in $UV$ light only.$R$ : This intercalating agent absorbs light in range $260-330 \; nm$.