Which of the following is the correct sequenc | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) The Polymerase Chain Reaction $(PCR)$ is a technique used to amplify a specific segment of $DNA$. It involves three main steps that are repeated i

Vedclass · Accepted Answer

Denaturation,Annealing,Extension

Vedclass · Answer

(C) The Polymerase Chain Reaction $(PCR)$ is a technique used to amplify a specific segment of $DNA$. It involves three main steps that are repeated in cycles:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (usually around $94-95^{\circ}C$) to separate the two strands.$2$. Annealing: The temperature is lowered (usually $50-65^{\circ}C$) to allow the primers to bind to the complementary sequences on the single-stranded $DNA$ templates.$3$. Extension: The temperature is adjusted (usually $72^{\circ}C$) for the $Taq$ $DNA$ polymerase enzyme to synthesize the new $DNA$ strand by adding nucleotides to the primers.Therefore,the correct sequence is Denaturation,Annealing,and Extension.

Which of the following is the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$?

Similar Questions

Which method is used to amplify a desired gene in large numbers?

You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.

The process of joining together different $DNA$ fragments is often referred to as

Explain how recombinant $DNA$ is introduced into a host cell or a living organism.

Select the incorrect statement regarding the continuous culture system.

Vedclass Test Series

Exam Paper Generator

Online Exam Module