What is the correct sequence of steps in the | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The process of $DNA$ fingerprinting involves several sequential steps:$1$. $DNA$ isolation: Extraction of $DNA$ from the sample.$2$. Restriction d

Vedclass · Accepted Answer

$DNA$ isolation $\rightarrow$ Restriction digestion $\rightarrow$ $PCR$ $\rightarrow$ Electrophoresis $\rightarrow$ Blotting $\rightarrow$ Autoradiography $\rightarrow$ Analysis

Vedclass · Answer

(B) The process of $DNA$ fingerprinting involves several sequential steps:$1$. $DNA$ isolation: Extraction of $DNA$ from the sample.$2$. Restriction digestion: $DNA$ is cut into fragments using restriction endonucleases.$3$. $PCR$ (Polymerase Chain Reaction): Amplification of specific $DNA$ sequences.$4$. Electrophoresis: Separation of $DNA$ fragments based on size.$5$. Blotting: Transferring the separated $DNA$ fragments onto a synthetic membrane (like nylon or nitrocellulose).$6$. Autoradiography: Hybridization with a radioactive probe and detection using $X$-ray film.$7$. Analysis: Comparing the banding patterns to identify individuals.Therefore,the correct sequence is $DNA$ isolation $\rightarrow$ Restriction digestion $\rightarrow$ $PCR$ $\rightarrow$ Electrophoresis $\rightarrow$ Blotting $\rightarrow$ Autoradiography $\rightarrow$ Analysis.

What is the correct sequence of steps in the process of $DNA$ fingerprinting?

Similar Questions

What is $DNA$ fingerprinting? Mention its application.

Would it be appropriate to use $DNA$ probes such as $VNTR$ in $DNA$ fingerprinting of a bacteriophage?

In $1980$,$F$. Sanger was awarded the Nobel Prize for the second time,sharing it with Gilbert and Maxam for their work on:

During $DNA$ fingerprinting,the $ds$-$DNA$ splits into $ss$-$DNA$ by . . . . . . treatment.

Vedclass Test Series

Exam Paper Generator

Online Exam Module