Which restriction enzymes have recognition si | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) In the cloning vector $pBR322$,the antibiotic resistance genes $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance) contain speci

Vedclass · Accepted Answer

$Pvu I, Pst I$ and $BamH I, Sal I$

Vedclass · Answer

(D) In the cloning vector $pBR322$,the antibiotic resistance genes $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance) contain specific recognition sites for restriction enzymes.$1$. The $amp^R$ gene contains recognition sites for the restriction enzymes $Pvu I$ and $Pst I$.$2$. The $tet^R$ gene contains recognition sites for the restriction enzymes $BamH I$ and $Sal I$.Therefore,the correct sequence is $Pvu I, Pst I$ for $amp^R$ and $BamH I, Sal I$ for $tet^R$.

Which restriction enzymes have recognition sites within the antibiotic resistance genes $amp^R$ and $tet^R$ in the plasmid $pBR322$?

Similar Questions

Assertion $A$: $DNA$ cannot pass through the cell membrane.
Reason $R$: $DNA$ is a hydrophobic molecule.

Two bacteria found to be very useful in genetic engineering experiments are

Where do restriction enzymes cut $DNA$ to produce blunt ends?

The introduction of $T-DNA$ into plants involves

Which antibiotic resistance is naturally found in $E. coli$?

Vedclass Test Series

Exam Paper Generator

Online Exam Module