$pBR322$ contains the recognition sites for which of the following endonucleases at the $amp^R$ site?

  • A
    $Pvu$ $II$ and $Pvu$ $I$
  • B
    $Pst$ $I$ and $Pvu$ $I$
  • C
    $Sal$ $I$ and $Bam$ $HI$
  • D
    $Pst$ $I$ and $Sal$ $I$

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The enzyme used during $\text{PCR}$ is$-$
$I.$ Taq polymerase
$II.$ Helicase
$III.$ $\text{RNA}$ polymerase
$IV.$ Gyrase
Select the correct option:

Which of the following statements does not hold true for restriction enzymes?

Where do restriction enzymes cut $DNA$ to produce blunt ends?

What is the function of $rop$ in a cloning vector?

An enzyme catalyzing the removal of nucleotides from the ends of $DNA$ is

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