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(B) The correct answer is $B$. Matthew Meselson and Franklin Stahl performed an experiment in $1958$ using $Escherichia 	ext{ } coli$ to prove that $

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Escherichia coli

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(B) The correct answer is $B$. Matthew Meselson and Franklin Stahl performed an experiment in $1958$ using $Escherichia 	ext{ } coli$ to prove that $DNA$ replication is semiconservative. They grew $E. 	ext{ } coli$ in a medium containing $^{15}N$ (a heavy isotope of nitrogen) for many generations, then transferred them to a medium with $^{14}N$ (a lighter isotope). By analyzing the density of the $DNA$ using cesium chloride density gradient centrifugation, they observed that the $DNA$ molecules were hybrids of both isotopes, confirming the semiconservative model of replication. This mechanism was subsequently shown to occur in higher organisms, including plants and human cells.

$DNA$ replicates semiconservatively was first shown in

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Ten $E. coli$ cells with $^{15}N$-dsDNA are incubated in a medium containing $^{14}N$ nucleotides. After $60$ minutes, how many $E. coli$ cells will have $DNA$ totally free from $^{15}N$ (in $cells$)?

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