E. coli bacteria grown in {}^{15}NH_4Cl mediu | vedclass

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(D) The correct answer is $D$. According to the Meselson and Stahl experiment,$E. coli$ cells grown in a medium containing $^{15}N$ (heavy isotope) in

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both hybrid and light $DNA$

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(D) The correct answer is $D$. According to the Meselson and Stahl experiment,$E. coli$ cells grown in a medium containing $^{15}N$ (heavy isotope) incorporate it into their $DNA$. When these cells are transferred to a medium containing $^{14}N$ (light isotope),the $DNA$ replication follows a semi-conservative model. After the $1^{st}$ generation ($20$ minutes),all $DNA$ molecules are hybrid $(^{15}N^{14}N)$. After the $2^{nd}$ generation ($40$ minutes),the hybrid $DNA$ molecules replicate to produce one hybrid $DNA$ molecule and one light $DNA$ molecule $(^{14}N^{14}N)$. Therefore,the resulting population contains both hybrid and light $DNA$ in equal proportions.

$E. coli$ bacteria grown in ${}^{15}NH_4Cl$ medium for several generations are allowed to grow in ${}^{14}NH_4Cl$ medium. After $2$ generations,the density gradient of the isolated $DNA$ is:

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