What type of vaccine is the Hepatitis $B$ vaccine?

In the process of recombinant $DNA$ technology,proteins and lipids are removed from the medium by which enzymes,respectively?

In $r-DNA$ technology,the term 'elution' refers to:

Following enzymes/chemical/technique are used in the process of gel electrophoresis:
$I.$ sample $DNA$ is cut into fragments
$II.$ restriction endonucleases
$III.$ agarose gel
$IV.$ ethidium bromide
$V.$ $UV$-radiation
$VI.$ elution
Mark the correct sequence of their use.

Arrange the steps of $\text{rDNA}$ technology in the correct order$:-$
$(I.)$ Extraction of the desired gene product.
$(II.)$ Amplification of the gene of interest.
$(III.)$ Isolation of the desired $\text{DNA}$ fragment.
$(IV.)$ Ligation of the $\text{DNA}$ fragment into a vector.
$(V.)$ Insertion of $\text{rDNA}$ into the host.
The correct order is$:-$

What is the first step in the Southern blot technique?