PCR and Restriction Fragment Length Polymorph | vedclass

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Question

(B) $PCR$ (Polymerase Chain Reaction) and $RFLP$ (Restriction Fragment Length Polymorphism) are fundamental techniques used in $DNA$ fingerprinting.$P

Vedclass · Accepted Answer

Genetic fingerprinting

Vedclass · Answer

(B) $PCR$ (Polymerase Chain Reaction) and $RFLP$ (Restriction Fragment Length Polymorphism) are fundamental techniques used in $DNA$ fingerprinting.$PCR$ is used to amplify small amounts of $DNA$ samples to obtain enough material for analysis.$RFLP$ involves cutting the $DNA$ with restriction enzymes to create fragments of varying lengths,which are then separated by gel electrophoresis to create a unique pattern for an individual.These techniques together allow for the identification of individuals based on their unique genetic makeup,which is the basis of genetic fingerprinting.

$PCR$ and Restriction Fragment Length Polymorphism $(RFLP)$ techniques are used for:

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