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(A) The experiment described is the Lederberg's replica plating experiment. $1$. Initially,bacterial colonies are grown on a master plate containing a

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Plates with and without streptomycin

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(A) The experiment described is the Lederberg's replica plating experiment. $1$. Initially,bacterial colonies are grown on a master plate containing a non-selective medium (without streptomycin). $2$. $A$ velvet-covered block is used to transfer the pattern of these colonies onto new plates (replica plates). $3$. Some of these replica plates contain streptomycin,while others do not. $4$. By comparing the growth on the streptomycin-containing plates with the original master plate,it is observed that the resistant colonies were already present in the original population before exposure to the antibiotic. $5$. This proves that mutations are pre-adaptive and do not arise as a direct response to the environment (streptomycin).

In the experiment to isolate streptomycin-resistant mutants using the replica plating technique,which proves that such mutations do not arise as an adaptation,what is required to be used for the replica plating?

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