Which of the following is NOT true for a clon | vedclass

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(A) cloning vector is a $DNA$ molecule used as a vehicle to artificially carry foreign genetic material into another cell. Key features of a cloning v

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Two origins of replication

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(A) cloning vector is a $DNA$ molecule used as a vehicle to artificially carry foreign genetic material into another cell. Key features of a cloning vector include: $1$. Origin of replication $(ori)$: This is the sequence where replication starts. $A$ vector should have only one $ori$ to control the copy number of the linked $DNA$. $2$. Selectable marker: Helps in identifying and eliminating non-transformants. $3$. Cloning sites: The vector should have very few,preferably single,recognition sites for commonly used restriction enzymes. Therefore,having 'two origins of replication' is not a characteristic of a standard cloning vector,as it would lead to instability and difficulty in controlling copy number. Thus,option $A$ is the correct answer.

Which of the following is $NOT$ true for a cloning vector?

Similar Questions

In which of the following are palindromic sequences present?

Assertion $A$: $DNA$ cannot pass through the cell membrane.
Reason $R$: $DNA$ is a hydrophobic molecule.

The gene of interest was cloned at the $Sal I$ site in $pBR322$. The recombinant plasmid will exhibit susceptibility to:

The first discovered restriction endonuclease that always cuts $DNA$ molecules at a particular point by recognizing a specific sequence of six base pairs is:

Which vector can clone small pieces of $DNA$?

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