PCR is a three-step process based on temperat | vedclass

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(A) $PCR$ (Polymerase Chain Reaction) involves three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx.

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Denaturation,Annealing,Extension

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(A) $PCR$ (Polymerase Chain Reaction) involves three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the single-stranded $DNA$ templates.$3$. Extension: The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ $DNA$ polymerase to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation,Annealing,and Extension.

$PCR$ is a three-step process based on temperature,which follows the sequence of ........

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