At what speed is a rotary shaker continuously | vedclass

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(C) In suspension culture, cells or cell aggregates are grown in a liquid medium. To ensure proper aeration and to prevent the cells from settling at

Vedclass · Accepted Answer

$100$ to $250\, rpm$

Vedclass · Answer

(C) In suspension culture, cells or cell aggregates are grown in a liquid medium. To ensure proper aeration and to prevent the cells from settling at the bottom, the culture flask is placed on a rotary shaker. The rotary shaker is typically operated at a speed of $100$ to $250\, rpm$ (revolutions per minute). This constant agitation helps in maintaining a uniform distribution of nutrients and oxygen throughout the medium.

Column-$I$	Column-$II$
$(i)$ Callus culture	$(A)$ Hybrid embryo recovery
$(ii)$ Suspension culture	$(B)$ Differentiated mass
$(iii)$ Embryo culture	$(C)$ Undifferentiated mass

At what speed is a rotary shaker continuously operated in suspension culture?

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Which of the following options represents the correct matching for the given columns?

Column-$I$ Column-$II$

$(i)$ Callus culture $(A)$ Hybrid embryo recovery

$(ii)$ Suspension culture $(B)$ Differentiated mass

$(iii)$ Embryo culture $(C)$ Undifferentiated mass

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