Which processes are used to develop new species in a botanical garden?

In somatic hybridization technique,the material generally used is

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

The part of the plant taken for tissue culture is called:

Somaclonal variation appears in plants:

What is the part of the plant used in tissue culture called?