$A$ - $amp^R$ has recognition sites for $Pvu I$.
$R$ - $tet^R$ gene has no recognition site for $Hind III$.

  • A
    $A$ and $R$ both are correct.
  • B
    $A$ and $R$ both are incorrect.
  • C
    $A$ is correct,$R$ is incorrect.
  • D
    $A$ is incorrect,$R$ is correct.

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Which of the following is used as a vector for cloning genes in higher organisms?

$pBR322$ was the first artificial cloning vector developed in $...A...$ by $...B...$ and $...C...$ from $E. coli$ plasmid. Here $A, B$ and $C$ can be:

Match Column-$I$ with Column-$II$ and choose the correct combination from the options given below:
Column-$I$ Column-$II$
$(a)$ Cloning vector $(i)$ Agrobacterium
$(b)$ Ti-$DNA$ $(ii)$ pBR$322$
$(c)$ Disarmed pathogen $(iii)$ $GAATTC$
$(d)$ Recognition sequence $(iv)$ Transformation

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