An antibiotic resistance gene in a vector hel | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) In recombinant $DNA$ technology,a vector (such as a plasmid) contains an antibiotic resistance gene (e.g.,$amp^R$ or $tet^R$) which acts as a sele

Vedclass · Accepted Answer

Both $(b)$ and $(c)$

Vedclass · Answer

(D) In recombinant $DNA$ technology,a vector (such as a plasmid) contains an antibiotic resistance gene (e.g.,$amp^R$ or $tet^R$) which acts as a selectable marker.$1$. When host cells are treated with the vector,only those cells that take up the plasmid become 'transformed'.$2$. These transformed cells can be selected by growing them on a medium containing the specific antibiotic; non-transformed cells will die.$3$. Furthermore,if the foreign $DNA$ is inserted into the antibiotic resistance gene,it causes 'insertional inactivation'. This allows for the differentiation between 'transformed' cells (containing the vector) and 'recombinant' cells (containing the vector with the foreign $DNA$ insert).$4$. Therefore,the antibiotic resistance gene is essential for selecting both transformed cells and identifying recombinant cells.

An antibiotic resistance gene in a vector helps in the selection of:

Similar Questions

'Cloning' is meant for

Identify the correct match for the given apparatus.
Apparatus $\to$ Function

What do '$S$' and '$T$' represent in the given figure of a stirred-tank bioreactor?

Identify the parts $A$ and $B$ from the given figure of the $pBR322$ cloning vector:

What color does $X-gal$ produce in the presence of $\beta$-galactosidase?

Vedclass Test Series

Exam Paper Generator

Online Exam Module