An isolated chloroplast,upon illumination,con | vedclass

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Question

(D) The process described is the Hill reaction,where light energy is used to split water (photolysis) to produce $O_2$ and reduce an electron acceptor

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Neither $O_2$ is produced nor ferric is reduced

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(D) The process described is the Hill reaction,where light energy is used to split water (photolysis) to produce $O_2$ and reduce an electron acceptor (ferric to ferrous). When an isolated chloroplast is washed in running water,the soluble components of the electron transport chain,such as plastocyanin $(PC)$,are removed or leached out. Since these components are essential for the flow of electrons from water to the electron acceptor,the entire process of light-dependent reactions is disrupted. Therefore,in the absence of these essential components,neither the photolysis of water (production of $O_2$) nor the reduction of ferric to ferrous can occur.

An isolated chloroplast,upon illumination,converts ferric into ferrous and produces $O_2$. What happens if it is washed in running water prior to illumination?

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