Assertion : Insertion of recombinant DNA with | vedclass

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(A) Alternative selectable markers have been developed to differentiate recombinants from non-recombinants based on their ability to produce color in

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If both Assertion and Reason are correct and the Reason is a correct explanation of the Assertion.

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(A) Alternative selectable markers have been developed to differentiate recombinants from non-recombinants based on their ability to produce color in the presence of a chromogenic substrate.When a recombinant $DNA$ is inserted into the coding sequence of the enzyme $\beta -$ galactosidase,the gene becomes non-functional.This process is known as insertional inactivation.Consequently,the bacteria containing the recombinant plasmid cannot produce the functional $\beta -$ galactosidase enzyme.As a result,they fail to produce color in the presence of a chromogenic substrate,leading to the formation of colorless colonies.Non-recombinant bacteria,which do not have the insert,produce functional $\beta -$ galactosidase and form blue-colored colonies.

Assertion : Insertion of recombinant $DNA$ within the coding sequence of $\beta -$ galactosidase results in colourless colonies.
Reason : Presence of insert results in inactivation of enzyme $\beta -$ galactosidase known as insertional inactivation.

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Assertion : Insertion of recombinant $DNA$ within the coding sequence of $\beta -$ galactosidase results in colourless colonies.Reason : Presence of insert results in inactivation of enzyme $\beta -$ galactosidase known as insertional inactivation.