Assertion : Restriction enzymes cut the stran | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) Restriction enzymes,a type of endonuclease,function by inspecting the length of a $DNA$ sequence. Once they find a specific recognition sequence,t

Vedclass · Accepted Answer

If the Assertion is correct but Reason is incorrect.

Vedclass · Answer

(C) Restriction enzymes,a type of endonuclease,function by inspecting the length of a $DNA$ sequence. Once they find a specific recognition sequence,they bind to it and cut each of the two strands of the double helix at specific points,leaving single-stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counterparts; i.e.,they can join similar complementary ends of $DNA$ fragments from other sources with the help of the enzyme $DNA$ ligase. Therefore,the stickiness of the ends facilitates the action of the enzyme $DNA$ ligase,not $DNA$ polymerase. Thus,the Assertion is correct,but the Reason is incorrect.

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

Similar Questions

The enzyme known as restriction endonuclease:

Restriction enzymes belong to which class of enzymes?

Recombinant $DNA$ is achieved by cleaving the pro-$DNA$ by

$DNA$ probes are copied from $mRNA$ molecules with the help of:

Hind $II$ always cuts $DNA$ molecules at a particular site called recognition sequence and it consists of : (in $bp$)

Vedclass Test Series

Exam Paper Generator

Online Exam Module

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.