Auxin can be bioassayed by

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(C) : Auxin bioassay is a quantitative test as it measures the concentration of auxin required to produce a specific effect and the magnitude of that

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Avena coleoptile curvature

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(C) : Auxin bioassay is a quantitative test as it measures the concentration of auxin required to produce a specific effect and the magnitude of that effect. The $Avena$ Curvature test is based on the experiments of Went $(1928)$. $A$ $10^{\circ}$ curvature is produced by an auxin concentration of $150 \ mg/L$ at $25^{\circ}C$ and $90\%$ relative humidity. This test can measure auxin concentrations up to $300 \ mg/L$. Auxin from a shoot tip or any other plant organ is allowed to diffuse into a standard-sized agar block (generally $2 	imes 2 	imes 1 \ mm$). Alternatively,auxin can be dissolved directly into the agar. $A$ $15-30 \ mm$ long oat coleoptile grown in the dark is held vertically over water. $A$ $1 \ mm$ tip of the coleoptile is removed without injuring the primary leaf. After $3$ hours,a second decapitation is performed at a distance of $4 \ mm$. The primary leaf is then pulled loose,and the agar block is placed against the tip of the decapitated coleoptile. After $90-110$ minutes,the coleoptile is observed to have bent. The curvature is then measured,or it can be photographed and determined via a shadow graph.

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