Bacterial colonies that have a foreign text{D | vedclass

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Question

(B) In the process of recombinant $	ext{DNA}$ technology,the $Lac \ Z$ gene encodes for the enzyme $\beta-$galactosidase. When a foreign $	ext{DNA}$

Vedclass · Accepted Answer

$\beta-$galactosidase gene shows insertional inactivation due to insertion of foreign $	ext{DNA}$

Vedclass · Answer

(B) In the process of recombinant $	ext{DNA}$ technology,the $Lac \ Z$ gene encodes for the enzyme $\beta-$galactosidase. When a foreign $	ext{DNA}$ fragment is inserted into this gene,it results in the disruption of the gene,a process known as insertional inactivation. As a result,the enzyme $\beta-$galactosidase is not produced. In the presence of a chromogenic substrate like $X-$gal,non-recombinant colonies (where the gene is intact) produce the enzyme,which cleaves $X-$gal to produce blue-colored colonies. Conversely,recombinant colonies (where the gene is inactivated) cannot produce the enzyme,so they cannot cleave $X-$gal and thus appear white.

Bacterial colonies that have a foreign $\text{DNA}$ fragment inserted into the plasmid will appear white,because $-$

Similar Questions

Identify the diagram given below.

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