Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign $DNA$ in addition to a selectable marker?

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(N/A) reporter enzyme is used to monitor the transformation of host cells by tracking the activity of its corresponding gene.
For example,the enzyme $\beta$-galactosidase $(LacZ)$ is used in blue-white screening.
When a foreign $DNA$ fragment is inserted into the $LacZ$ gene,the gene becomes inactivated (insertional inactivation).
Consequently,the transformed cells do not produce $\beta$-galactosidase and appear white in the presence of a chromogenic substrate.
In contrast,non-transformed cells or cells with non-recombinant plasmids produce the enzyme and appear blue.

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Similar Questions

What happens if a foreign $DNA$ fragment is inserted into the coding sequence of the $\beta$-galactosidase enzyme in a plasmid containing the coding sequence for the $\beta$-galactosidase enzyme?

Explain how recombinant $DNA$ is introduced into a host cell or a living organism.

Match List $I$ with List $II$:
List $I$List $II$
a) Visualization of $DNA$ in gel electrophoresisi) Chitinase
b) Precipitation of $DNA$ii) Lysozyme
c) Breaking of cell wall of bacteriaiii) Chilled ethanol
d) Breaking of cell wall of fungusiv) Ethidium bromide

Codes:

Recombinant $\text{DNA}$ is directly injected into the nucleus of an animal cell by $-$

Select the correct sequence of steps in $PCR$.

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