Enzymes can be immobilised by:

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(D) Enzyme immobilisation is a technique used to restrict the mobility of enzymes to a fixed space. $(a)$ Cross-linking involves binding enzyme molecu

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All the above

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(D) Enzyme immobilisation is a technique used to restrict the mobility of enzymes to a fixed space. $(a)$ Cross-linking involves binding enzyme molecules to each other using bifunctional or multifunctional reagents. $(b)$ Covalent attachment involves forming stable chemical bonds between the enzyme and a solid support matrix. $(c)$ Entrapment involves trapping enzymes within a polymer matrix or gel (like alginate or polyacrylamide) while allowing substrates to diffuse in and products to diffuse out. Since all these methods are standard techniques for enzyme immobilisation,the correct answer is $D$.

Substrate	Enzyme
$(A)$ Ribonucleotide	$(i)$ Chitinase
$(B)$ Chitin	$(ii)$ Cellulase
$(C)$ Cellulose	$(iii)$ Ribonuclease

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Match the following substrates with their respective enzymes:

Substrate Enzyme

$(A)$ Ribonucleotide $(i)$ Chitinase

$(B)$ Chitin $(ii)$ Cellulase

$(C)$ Cellulose $(iii)$ Ribonuclease

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