Hardening in tissue culture is

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(B) Hardening is the process of gradual acclimatization of plantlets produced through tissue culture to the external environment. These plantlets are

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Acclimatization of tissue culture plants slowly before growing in the field

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(B) Hardening is the process of gradual acclimatization of plantlets produced through tissue culture to the external environment. These plantlets are initially grown in a controlled,high-humidity,and low-light environment. Before transferring them to the field,they are exposed to natural conditions slowly to ensure they can survive and adapt to the new environment.

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