Identify the palindromic nucleotide sequence.

The gene whose expression helps to identify the transformed cell is known as........

Given below are two statements:
Statement $I:$
Restriction endonucleases recognise specific sequences to cut $DNA$,known as palindromic nucleotide sequences.
Statement $II:$
Restriction endonucleases cut the $DNA$ strand a little away from the centre of the palindromic site.
In the light of the above statements,choose the most appropriate answer from the options given below:

What is required for the functioning of a cloning vector $DNA$?

What is $pBR322$?

Match Column-$I$ with Column-$II$ and choose the correct combination from the options given below:

Column-$I$	Column-$II$
$(a)$ Cloning vector	$(i)$ Agrobacterium
$(b)$ Ti-$DNA$	$(ii)$ pBR$322$
$(c)$ Disarmed pathogen	$(iii)$ $GAATTC$
$(d)$ Recognition sequence	$(iv)$ Transformation