If a probe is made of radioactively labelled | vedclass

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Question

(A) probe is typically a single-stranded $DNA$ or $RNA$ molecule tagged with a radioactive isotope. In the given question,the probe is described as $d

Vedclass · Accepted Answer

It is double-stranded $DNA$.

Vedclass · Answer

(A) probe is typically a single-stranded $DNA$ or $RNA$ molecule tagged with a radioactive isotope. In the given question,the probe is described as $dsDNA$ (double-stranded $DNA$). Double-stranded $DNA$ cannot hybridise directly to its complementary sequence because the strands are already base-paired with each other. Therefore,the statement that the probe is $dsDNA$ is incorrect,as probes must be single-stranded to allow for hybridisation with the target sequence.

If a probe is made of radioactively labelled $dsDNA$ and is allowed to hybridise to its complementary $DNA$ in a cloned cell followed by detection using autoradiography,which of the following statements is considered wrong?

Similar Questions

Who discovered $DNA$ fingerprinting?

$DNA$ fingerprinting is useful for:

Choose the correct option for a molecular probe.
$I -$ Long chain of nucleic acid
$II -$ Double-stranded
$III -$ Single-stranded
$IV -$ Short chain of nucleic acid

Who developed $DNA$ fingerprinting?

When the genomes of two people are cut using the same restriction enzyme,the length and number of fragments obtained are different; this is called:

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