In DNA fingerprinting technique,the radioacti | vedclass

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Question

(B) In the $DNA$ fingerprinting technique,the process involves Southern blotting where $DNA$ fragments are separated by gel electrophoresis and transf

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Single-stranded $DNA$

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(B) In the $DNA$ fingerprinting technique,the process involves Southern blotting where $DNA$ fragments are separated by gel electrophoresis and transferred to a membrane. These $DNA$ fragments are then denatured to form single-stranded $DNA$. During the hybridization step,a radioactive probe (which is a single-stranded $DNA$ sequence) is added. This probe binds to its complementary sequence on the membrane,forming a double-stranded $DNA$ hybrid. However,the intermediate state or the specific molecules involved in the hybridization process that are detected are the single-stranded $DNA$ probes binding to the target $DNA$. Specifically,the question refers to the nature of the radioactive probe used for hybridization,which is a single-stranded $DNA$ molecule.

In $DNA$ fingerprinting technique,the radioactive intermediates formed during hybridization are . . . . . . molecules.

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