In order to obtain disease-free plants through tissue culture methods,the best technique is:

Which of the following is used as an explant in tissue culture?

After the demonstration of totipotency,a botanist wishes to produce identical plants from an angiosperm by tissue culture. The parts or tissues which are likely to yield haploid embryos are:

Somaclones are obtained by

Virus-free plants can be obtained through $-$

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below: