In the technique of DNA fingerprinting,digest | vedclass

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Question

(A) The technique of $DNA$ fingerprinting involves several steps: isolation of $DNA$,digestion of $DNA$ by restriction endonucleases,separation of $DN

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Electrophoresis

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(A) The technique of $DNA$ fingerprinting involves several steps: isolation of $DNA$,digestion of $DNA$ by restriction endonucleases,separation of $DNA$ fragments by gel electrophoresis,transferring (blotting) of separated $DNA$ fragments to synthetic membranes (like nitrocellulose or nylon),hybridization using labeled $VNTR$ probe,and detection of hybridized $DNA$ fragments by autoradiography.Therefore,after the digestion of $DNA$ by restriction enzymes,the next step is the separation of these fragments based on their size using gel electrophoresis.

In the technique of $DNA$ fingerprinting,digestion of $DNA$ is followed by:

Similar Questions

$DNA$ fingerprinting was discovered by .........

$A$: Unknown $DNA$ after hybridization with $VNTR$ probe,the autoradiogram gives many bands of differing sizes in $DNA$ profiling.
$R$: These bands represent the $DNA$ fingerprint of the organism.

Nowadays,it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize with its complementary $DNA$ in a clone of cells,followed by its detection using autoradiography because:

Which of the following works on the principle of the method developed by Frederick Sanger?

$DNA$ fingerprinting method is very useful for:

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