The Michaelis constant (K_m) value of an enzy | vedclass

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(C) The Michaelis constant $(K_m)$ is defined as the substrate concentration at which the reaction rate (velocity) is exactly half of the maximum velo

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$1/2 \; V_{max}$

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(C) The Michaelis constant $(K_m)$ is defined as the substrate concentration at which the reaction rate (velocity) is exactly half of the maximum velocity $(V_{max})$. According to the Michaelis-Menten equation: $v = \frac{V_{max} [S]}{K_m + [S]}$. When $[S] = K_m$,the equation becomes $v = \frac{V_{max} K_m}{K_m + K_m} = \frac{V_{max} K_m}{2 K_m} = \frac{V_{max}}{2}$. Therefore,$K_m$ represents the substrate concentration required to achieve half of the maximum reaction velocity.

The Michaelis constant $(K_m)$ value of an enzyme is the substrate concentration at which the velocity of the reaction is:

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