Restriction enzymes that are used in the cons | vedclass

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(B) Restriction enzymes,specifically Type $II$ endonucleases,recognize and cut $DNA$ at specific palindromic sequences. This process generates fragmen

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The $DNA$ fragments would not have complementary sticky ends,making recombinant $DNA$ construction impossible.

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(B) Restriction enzymes,specifically Type $II$ endonucleases,recognize and cut $DNA$ at specific palindromic sequences. This process generates fragments with single-stranded overhangs known as 'sticky ends'. These sticky ends are crucial because they allow the $DNA$ fragment to base-pair with a complementary vector $DNA$ cut by the same enzyme. If the enzymes cut at random sites,the resulting fragments would lack these specific sticky ends,making it impossible to join the gene of interest with the vector to form a recombinant $DNA$ molecule.

Restriction enzymes that are used in the construction of recombinant $DNA$ are endonucleases which cut the $DNA$ at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the $DNA$ at specific-recognition sequence?

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