Significance of heat shock method in bacteria | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) The heat shock method is a technique used in genetic engineering to introduce recombinant $DNA$ into bacterial cells (like $E. coli$).Bacteria are

Vedclass · Accepted Answer

Uptake of $DNA$ through transient pores in the bacterial cell wall

Vedclass · Answer

(C) The heat shock method is a technique used in genetic engineering to introduce recombinant $DNA$ into bacterial cells (like $E. coli$).Bacteria are generally not capable of taking up foreign $DNA$ directly from their environment.To make them 'competent',they are treated with a divalent cation (like $Ca^{2+}$),which increases the efficiency with which $DNA$ enters the bacterium through pores in its cell wall.During the heat shock process,the cells are incubated with recombinant $DNA$ on ice,then placed briefly at $42^{\circ}C$ (heat shock),and finally put back on ice.This sudden temperature change creates transient pores in the bacterial cell wall,allowing the recombinant $DNA$ to enter the cell.

Significance of heat shock method in bacterial transformation is to facilitate

Similar Questions

In the purification process of recombinant $DNA$ technology,what does the addition of chilled ethanol precipitate?

$RNA$ is removed by the treatment with

The cutting of $DNA$ by restriction endonucleases results in the fragments of $DNA$. These fragments can be separated by a technique known as ......

In a mixture,$DNA$ fragments are separated by

In Southern blotting,which of the following is separated by gel electrophoresis?

Vedclass Test Series

Exam Paper Generator

Online Exam Module