The Polyethylene text{ } Glycol (PEG) method | vedclass

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Question

(B) $Polyethylene 	ext{ } Glycol$ $(PEG)$ is a chemical agent used in plant tissue culture and biotechnology to facilitate the direct uptake of forei

Vedclass · Accepted Answer

Gene transfer without a vector

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(B) $Polyethylene 	ext{ } Glycol$ $(PEG)$ is a chemical agent used in plant tissue culture and biotechnology to facilitate the direct uptake of foreign $DNA$ into protoplasts. This process is known as direct gene transfer or vectorless gene transfer, as it does not require a biological vector like $Agrobacterium 	ext{ } tumefaciens$ to introduce the genetic material into the host cell.

The $Polyethylene \text{ } Glycol$ $(PEG)$ method is used for:

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