The characteristics of a molecular probe are
$I.$ very long molecule
$II.$ double-stranded
$III.$ $DNA$ or $RNA$
$IV.$ complementary to a part of the desired gene
The correct pair is

  • A
    $I$ and $II$
  • B
    $II$ and $III$
  • C
    $III$ and $IV$
  • D
    $IV$ and $I$

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$I.$ $Eco RI$ restriction endonuclease enzyme is isolated from $Escherichia \; coli \; RY13$.
$II.$ Its recognition sequence is $5^{\prime}-GAATTC-3^{\prime}$ / $3^{\prime}-CTTAAG-5^{\prime}$.
$III.$ Its site of cleavage is between $G$ and $A$ in the sequence $5^{\prime}-GAATTC-3^{\prime}$.

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$A$ chimaeric $DNA$ is formed by

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