Which of the following cells cannot be grown under tissue culture conditions?

During tissue culture,the growth hormones are provided to callus to induce . . . . . . .

Given below are two statements:
Statement $I$: In suspension culture and callus culture technique,subculturing is necessary.
Statement $II$: Subculturing is needed only in suspension culture technique.
In the light of above statements,select the correct option given below:

In which of the following methods can plantlets be developed from seeds that remain dormant for a long time?

From which plant were haploid plants first obtained through anther culture?

Virus-free plants can be formed by