The given figure is the diagrammatic representation of the $E. coli$ vector $pBR322$. Which one of the given options correctly identifies its certain component$(s)$?
- A$Ori$ - original restriction enzymes
- B$Rop$ - reduced osmotic pressure
- C$Hind III, Eco RI$ - selectable markers
- D$amp^R, tet^R$ - antibiotic resistance genes
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Plasmid of which bacterium was first time used in recombinant $DNA$ technology?
MediumAIIMS 2019
View SolutionRestriction in Restriction endonuclease enzyme refers to
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View SolutionThe discovery of which of the following has made it possible to cut $DNA$ at specific locations?
Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?
The restriction enzyme responsible for the cleavage of the following sequence is:
$5^{\prime}-G-T-C-G-A-C-3^{\prime}$
$3^{\prime}-C-A-G-C-T-G-5^{\prime}$
$5^{\prime}-G-T-C-G-A-C-3^{\prime}$
$3^{\prime}-C-A-G-C-T-G-5^{\prime}$
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