The International Centre for Genetic Engineering and Biotechnology $(ICGEB)$ established by the United Nations Organization $(UNO)$ is located at

Which of the following is/are correct about Adenosine Deaminase $(ADA)$ deficiency?
$I$. In the absence of adenosine deaminase enzyme,purine metabolism is disturbed and $T$-lymphocytes fail to function.
$II$. $ADA$ deficiency is caused by the deletion of the gene for $ADA$.
$III$. In some cases,it can be cured by bone marrow transplantation and enzyme replacement therapy. But in both approaches,the patients are not completely cured.
$IV$. For permanent cure,genes isolated from the bone marrow cells producing $ADA$ at early embryonic stages can be a possible cure.
Which of the above statements are correct?

In transgenics,the expression of transgene in the target tissue is known by:

The two polypeptides of human insulin are linked together by

The vaccine of Hepatitis-$B$ is a

Given below is the sequence of events in the production of human insulin by Eli Lilly.
$(a)$ Preparation of two $DNA$ sequences corresponding to chain $A$ and $B$ of human insulin.
$(b)$ Production of chain $A$ and $B$ separately.
$(c)$ Introduction of chains in plasmids of $E. coli$.
$(d)$ Extraction of chain $A$ and $B$ and combining them by creating disulphide bonds.
Choose the correct sequence of events.