What is the effect of substrate concentration on the rate of reaction?

(N/A) The effect of substrate concentration on the rate of reaction is as follows:
$1$. Initially,as the substrate concentration increases,the velocity of the enzymatic reaction increases.
$2$. The reaction eventually reaches a maximum velocity,denoted as $V_{\max}$.
$3$. Beyond this point,any further increase in substrate concentration does not increase the reaction rate.
$4$. This occurs because,at high concentrations,all available enzyme active sites are occupied by substrate molecules,leading to saturation. There are no free enzyme molecules left to bind with additional substrate molecules.